Optimization of Apolipoprotein(a) Genotyping with Pulsed Field Gel Electrophoresis

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Optimization of apolipoprotein(a) genotyping with pulsed field gel electrophoresis.

BACKGROUND Increased lipoprotein(a) is a risk factor for atherosclerosis, and its concentration in serum is inversely correlated with the size of the apoliprotein(a) [apo(a)] component. The size of the apo(a) gene is determined mainly by the Kringle IV size polymorphism. We have optimized and characterized pulsed field gel electrophoresis (PFGE) for apo(a) genotyping. METHODS Established PFGE...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1999

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/45.6.771